High Speed Atomic Force Microscopy of Biomolecules by Image Tracking
نویسندگان
چکیده
منابع مشابه
High-speed atomic force microscopy.
High-speed atomic force microscopy (HS-AFM) has been developed as a nano-dynamics visualization technique. This microscopy permits direct observation of structure dynamics and dynamic processes of biological molecules in physiological solutions, at a subsecond to sub-100 ms temporal resolution and an ∼2 nm lateral and a 0.1 nm vertical resolution. Importantly, tip-sample interactions do not dis...
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Many applications in materials science, life science and process control would benefit from atomic force microscopes (AFM) with higher scan speeds. To achieve this, the performance of many of the AFM components has to be increased. In this work, we focus on the cantilever sensor, the scanning unit and the data acquisition. We manufactured 10 microm wide cantilevers which combine high resonance ...
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A key hurdle in achieving high scan speeds in atomic force microscopes is that the probe is required to be scanned over the sample in a zig-zag raster pattern. The fast axis of the AFM scanner must track a signal that contains frequencies beyond its mechanical bandwidth. Consequently, fast raster scans generate distortions in the resulting image. We propose a smooth cycloid-like scan pattern th...
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In this paper, a simple and reproducible approach for attaching the multi-wall carbon nanotubes (MWNTs) to the apex of the atomic force microscope probe has been proposed. For this purpose, the dielectrophoresis method was applied due to its simple performance, cheapness and reliability. In this method, various parameters such as voltage, frequency, concentration of carbon nanotubes solution an...
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High-speed imaging by atomic force microscopy (AFM) requires the implementation of a fast control of tip–surface distance to maintain a constant interaction force. In particular, a well-controlled low load force is essential for observing soft biological molecules without causing damage to the sample. The accurate control of tip–surface distance where only feedback control is used is intrinsica...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1999
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(99)77068-2